x-ray crystal structure of the protein Search Results


xrcc4  (Bioss)
94
Bioss xrcc4
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
Xrcc4, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/x-ray+crystal+structure+of+the+protein/XRCC4+Polyclonal+Antibody/pmc11405603-85-59-66
Average 94 stars, based on 1 article reviews
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monoclonal antibody  (Boster Bio)
90
Boster Bio monoclonal antibody
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/x-ray+crystal+structure+of+the+protein/Anti-Ku-80+XRCC5+Monoclonal+Antibody/pmc03406497-44-18-20
Average 90 stars, based on 1 article reviews
monoclonal antibody - by Bioz Stars, 2026-10
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x rad 320 biological irradiator  (Precision X-Ray)
98
Precision X-Ray x rad 320 biological irradiator
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
X Rad 320 Biological Irradiator, supplied by Precision X-Ray, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
x rad 320 biological irradiator - by Bioz Stars, 2026-10
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x-ray super rx film 100 nif 18 × 24  (FUJIFILM)
90
FUJIFILM x-ray super rx film 100 nif 18 × 24
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
X Ray Super Rx Film 100 Nif 18 × 24, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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x-ray super rx film 100 nif 18 × 24 - by Bioz Stars, 2026-10
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x-ray film  (FUJIFILM)
90
FUJIFILM x-ray film
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
X Ray Film, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
x-ray film - by Bioz Stars, 2026-10
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anti-fanca  (GeneTex)
90
GeneTex anti-fanca
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
Anti Fanca, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xrcc4 xrcc4 gtx83406 antibody  (GeneTex)
90
GeneTex xrcc4 xrcc4 gtx83406 antibody
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
Xrcc4 Xrcc4 Gtx83406 Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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x ray film  (Bio-Rad)
96
Bio-Rad x ray film
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
X Ray Film, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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x ray film - by Bioz Stars, 2026-10
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x ray film over assay kit  (Bio-Rad)
97
Bio-Rad x ray film over assay kit
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
X Ray Film Over Assay Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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x ray film over assay kit - by Bioz Stars, 2026-10
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chemidoc x ray spectrometry xrs imager  (Bio-Rad)
96
Bio-Rad chemidoc x ray spectrometry xrs imager
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
Chemidoc X Ray Spectrometry Xrs Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemidoc x ray spectrometry xrs imager - by Bioz Stars, 2026-10
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rat anti mouse cd16 32 clone 2 4g2  (Bio X Cell)
98
Bio X Cell rat anti mouse cd16 32 clone 2 4g2
Cellular distribution and relative levels of <t>XRCC4</t> protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001
Rat Anti Mouse Cd16 32 Clone 2 4g2, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xrcc4  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology xrcc4
( A ) ParC5 shNT and shδ110 NHEJ reporter cells were generated that stably express shSCR, shSIRT6–1, shSIRT6–2, or shSIRT6–3. Lysates were immunoblotted for the indicated proteins. ( B ) Top: Histone fractions from ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were acid-extracted and immunoblotted for H3K36me2 and total H3. Bottom: Densitometry of H3K36me2 normalized to total H3. ( C ) MNase assay was performed on ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells. Percentage of DNA in each [NaCl] fraction was assayed as described in . ( D ) DNA repair was analyzed in ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 NHEJ reporter cells and quantified as described in . ( E to H ) Lysates from ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were immunoblotted with the indicated antibodies. Densitometry of DNA Lig4 (F), <t>XRCC4</t> (G), and XLF (H) normalized to their corresponding β-actin control from the Western blots shown in (E). ( I ) ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were IR at 10 Gy and allowed to recover for 48 hrs. Cells were harvested and caspase-3 activity was assayed. Shown is the fold induction of caspase-3 activities in each cell line over their own corresponding no IR controls. Shown is data from at least three independent biological replicates. Data is shown as mean ± SEM. Statistics represent two-way ANOVA followed by Tukey’s multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
Xrcc4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellular distribution and relative levels of XRCC4 protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Journal of Assisted Reproduction and Genetics

Article Title: The DNA double-strand break repair proteins γH2AX, RAD51, BRCA1, RPA70, KU80, and XRCC4 exhibit follicle-specific expression differences in the postnatal mouse ovaries from early to older ages

doi: 10.1007/s10815-024-03189-4

Figure Lengend Snippet: Cellular distribution and relative levels of XRCC4 protein in the postnatal mouse ovaries. a Representative microscopic micrographs of XRCC4 immunostaining of prepuberty (PreP, n = 6), puberty (P, n = 7), postpuberty (PostP, n = 7), early aged (EA, n = 7), and late aged (LA, n = 7) groups. XRCC4 protein was intensely expressed in oocytes and granulosa cells of the follicles at different developmental stages as well as in granulosa lutein and theca lutein cells. The asterisks indicate small spaces between granulosa cells, the red arrows show germinal epithelium. The micrographs and their inserts were captured at 200 × and 400 × original magnifications, respectively. Scale bars represent 50 µm. O, Oocyte; TL, Theca layer; S, Stroma; SF, Secondary follicle; PAF, Preantral follicle; AF, Antral follicle; AtF, Atretic follicle; CL, Corpus luteum. b The relative XRCC4 protein levels in the total area. It gradually increased from the prepuberty to the late aged groups ( P < 0.05). c-h The relative XRCC4 protein levels in c primordial, d primary, e secondary, f preantral, g antral, and h atretic follicles, and in oocyte’s cytoplasm (C) and nucleus (N), granulosa cells, and theca cells. Although we found no changes in primordial and atretic follicles, there was an increasing trend of XRCC4 expression in primary, secondary, preantral, and antral follicles toward to the early or late aged group when compared to the early groups ( P < 0.05). i The relative XRCC4 protein levels in ovarian cells located in the germinal epithelium, stroma, and corpus luteum. It increased in stromal cells, granulosa lutein and theca lutein cells from the prepuberty to the late aged groups ( P < 0.01). Data were analyzed using one-way ANOVA and Tukey’s post hoc test and are presented as the mean ± standard deviation (SD). Asterisks above the columns indicate significant differences as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: Next, the sections were incubated with γH2AX (diluted 1:1000; catalog no. 9718S, Cell Signaling Technology, Danvers, MA, USA), RAD51 (diluted 1:250; catalog no. bs-20297R, Bioss Inc., Woburn, MA, USA), BRCA1 (diluted 1:1000; catalog no. bs-0803R, Bioss Inc.), and RPA70 (diluted 1:3000; catalog no. 2267S, Cell Signaling Technology), KU80 (diluted 1:250; catalog no. MBS712948, MyBiosource, Inc., San Diego, CA, USA), XRCC4 (diluted 1: 250; catalog no. bs8510R, Bioss Inc.), cCASP3 (diluted 1:300; catalog no. 9661S, Cell Signaling Technology), β-GAL (diluted 1:1300; catalog no. 11518–1-AP, Proteintech Group, Rosemont, IL, USA) primary antibodies at + 4 °C overnight.

Techniques: Immunostaining, Expressing, Standard Deviation

( A ) ParC5 shNT and shδ110 NHEJ reporter cells were generated that stably express shSCR, shSIRT6–1, shSIRT6–2, or shSIRT6–3. Lysates were immunoblotted for the indicated proteins. ( B ) Top: Histone fractions from ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were acid-extracted and immunoblotted for H3K36me2 and total H3. Bottom: Densitometry of H3K36me2 normalized to total H3. ( C ) MNase assay was performed on ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells. Percentage of DNA in each [NaCl] fraction was assayed as described in . ( D ) DNA repair was analyzed in ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 NHEJ reporter cells and quantified as described in . ( E to H ) Lysates from ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were immunoblotted with the indicated antibodies. Densitometry of DNA Lig4 (F), XRCC4 (G), and XLF (H) normalized to their corresponding β-actin control from the Western blots shown in (E). ( I ) ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were IR at 10 Gy and allowed to recover for 48 hrs. Cells were harvested and caspase-3 activity was assayed. Shown is the fold induction of caspase-3 activities in each cell line over their own corresponding no IR controls. Shown is data from at least three independent biological replicates. Data is shown as mean ± SEM. Statistics represent two-way ANOVA followed by Tukey’s multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Journal: bioRxiv

Article Title: PKCδ regulates chromatin remodeling and DNA repair through SIRT6

doi: 10.1101/2023.05.24.541991

Figure Lengend Snippet: ( A ) ParC5 shNT and shδ110 NHEJ reporter cells were generated that stably express shSCR, shSIRT6–1, shSIRT6–2, or shSIRT6–3. Lysates were immunoblotted for the indicated proteins. ( B ) Top: Histone fractions from ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were acid-extracted and immunoblotted for H3K36me2 and total H3. Bottom: Densitometry of H3K36me2 normalized to total H3. ( C ) MNase assay was performed on ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells. Percentage of DNA in each [NaCl] fraction was assayed as described in . ( D ) DNA repair was analyzed in ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 NHEJ reporter cells and quantified as described in . ( E to H ) Lysates from ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were immunoblotted with the indicated antibodies. Densitometry of DNA Lig4 (F), XRCC4 (G), and XLF (H) normalized to their corresponding β-actin control from the Western blots shown in (E). ( I ) ParC5 shNT/shδ110-shSCR/shSIRT6–2/shSIRT6–3 cells were IR at 10 Gy and allowed to recover for 48 hrs. Cells were harvested and caspase-3 activity was assayed. Shown is the fold induction of caspase-3 activities in each cell line over their own corresponding no IR controls. Shown is data from at least three independent biological replicates. Data is shown as mean ± SEM. Statistics represent two-way ANOVA followed by Tukey’s multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Antibodies for DNA Ligase IV (Cat # sc-271299, RRID:AB_10610371), XRCC4 (Cat # sc-271087, RRID:AB_10612396), XLF (Cat # sc-166488, RRID:AB_2152940), SIRT6 (Cat # sc-517556, RRID:AB_2915920), and Mre11 (Cat # sc-135992, RRID:AB_2145244) were purchased from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Generated, Stable Transfection, Control, Western Blot, Activity Assay